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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

                                                

                                                        

                                
品牌：Cayman

                            

                                                        
                        

                    

                    

                        

                                                                                    

                                货号：500224-2400 wells
                                规格：2400 wells
                                目录价：￥79472
                            

                                                        

                                货号：500224-480 wells
                                规格：480 wells
                                目录价：￥18736
                            

                                                        

                                货号：500224-4800 wells
                                规格：4800 wells
                                目录价：￥98784
                            

                                                        

                                货号：500224-96 wells
                                规格：96 wells
                                目录价：￥5968
                            

                                                        

                                货号：500224-9600 wells
                                规格：9600 wells
                                目录价：￥122896
                            

                                                                                

                    

                    
产品详情

                    

                                                

                            
产品名称:

                            
THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

                        

                                                                        

                            
别名:

                            
eIF4E-binding Protein 1|PHAS-I|Phosphorylated Heat- and Acid-Stable Protein Regulated by Insulin 1

                        

                                                                        

                            
产品描述:

                            
 The Phospho-4EBP1 (T37/T46) assay kit is a homogeneous time_x0002_resolved Förster resonance energy transfer (TR-FRET) sandwich immunoassay. The THUNDER™ Cell Signaling assay workflow consists of 3 steps. Following cell treatment, cells are first lysed with the specific Lysis Buffer provided in the kit. Then Phospho-4EBP1 (T37/T46) in the cell lysates is detected with a pair of fluorophore-labeled antibodies in a simple "add-incubate measure" format (single-step reagent addition; no wash steps). One antibody is labeled with a donor fluorophore (Europium chelate; Eu-Ab1) and the second with a far-red acceptor fluorophore (FR-Ab2). The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two dyes into close proximity. Excitation of the donor Europium chelate molecules with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a FRET from the donor to the acceptor molecules, which in turn emit a TR-FRET signal at 665 nm. Residual energy from the Eu chelate generates light at 615 nm. The signal at 665 nm is proportional to the concentration of Phospho-4EBP1 (T37/T46) in the cell lysate. Data can be expressed as either the signal at 665 nm or the 665 nm/615 nm ratio.

                        

                                                                                                                                                                                                                                                                                                                                                                                                                                                

                            
保存温度:

                            
-80

                        

                                                                        

                            
运输温度:

                            
-80

                        

                                                                                                                                                                        

                            
有效期:

                            
180

                        

                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                                        

                        

                            

                                                                

                                    

                                

                                                            

                        

                        

                        

                    

                                        
                                        
                                    

                
            

        

    

    

    

        

            

            

                

                    
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