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折扣/货期| 货号：SBB-KF0120-KI01 | 规格：1 Kit | 目录价：￥14168 |
产品详情
产品名称:
Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit
产品描述:
South Bay Bio’s Cullin4a/Rbx1/DDB1/CRBN Neddylated E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto the E3 complex in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto CRBN (or substrates) in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
靶标/特异性:
Readout: Endpoint/Kinetic Label or Dye: Europium-Cryptate & Cy5 Detection Method: TR-FRET Substrate Properties: Protein-based Substrate. Typical experimental concentration is 1x. Excitation/Emission (nm): 304/620 & 304/665
保存温度:
-80°C
运输温度:
Dry Ice
产地:
瑞士
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