PEDF (human, recombinant)

Cell Proliferation-Inducing Gene 35 Protein|Early Population Doubling Level cDNA-1|EPC-1|Pigment Epithelium-Derived Factor|Serpin F1

Pigment epithelium-derived factor (PEDF) is a secreted glycoprotein encoded by the SERPINF1 gene.{54180} It is a non-inhibitory member of the serine-protease inhibitor (SERPIN) superfamily that has anti-angiogenic and neurotrophic activities. PEDF is a 418-amino acid protein comprised of an N-terminal region important for its neurotrophic activity, three β-sheets and ten α-helices that make up an α/β core serpin domain, and a C-terminal region containing a glycosylation site.{54180,54181} It has an asymmetrical charge distribution with the basic region involved in glycosaminoglycan binding and the acidic region binding to collagen I.{54181} PEDF is ubiquitously expressed and localized to the extracellular matrix but can also be found in the cytoplasm and nucleus of retinal pigment epithelial cells and certain cancer cell lines.{54180,54182} It contains biologically active peptides, including a 34-amino acid peptide (amino acids 44-77), which contains a laminin receptor binding site and has anti-angiogenic, bone mineralization, and apoptotic activities, and a 44-amino acid peptide (amino acids 78-121), which contains a PNPLA2 binding site and promotes neuronal differentiation and inhibits vascular permeability.{54180,54183,54184} PEDF also interacts with collagen I for its anti-angiogenic activity, as well as with sulfated (heparin) and non-sulfated (hyaluronan) glycosaminoglycans, which are important for PNPLA2 receptor binding and activation of caspases, respectively.{54180,54181} Its expression is reduced in a variety of cancer cell lines and solid tumors, and the reduction is associated with metastasis and poor prognosis.{54181} Mutations in SERPINF1 are responsible for the hereditary bone dysplasia disorder osteogenesis imperfecta type VI, which is characterized by bone fragility, deformity, and growth deficiency.{54185} Cayman’s PEDF (human, recombinant) protein consists of 410 amino acids, has a calculated molecular weight of 45.8 kDa, and a predicted N-terminus of Gln20 after signal peptide cleavage.

-80

-80

Lyophilized from sterile PBS, pH 7.4

365

≥98% estimated by SDS-PAGE