HGF (human, recombinant)

Hepatocyte Growth Factor|Hepatopoietin-A|HPTA|Lung Fibroblast-derived Mitogen|Scatter Factor

Hepatocyte growth factor (HGF) is produced by cells of mesenchymal origin and has roles in cell proliferation, survival, motility, and morphogenesis.{53757,53758} HGF encodes the inactive polypeptide chain pre-pro-HGF that is composed of a signal sequence (1-31), a 69 kDa heavy α chain (32-494), and a 34 kDa light β chain (495-728).{53758} Degradation of the signaling peptide produces pro-HGF which is also cleaved between Arg494 and Val495, primarily by the protease HGF activator (HGF-A), to release the α and β chains. The free α and β chains then form an active heterodimer via disulfide bonding of cysteine residues that binds to the MET receptor to induce intracellular signaling. Transgenic overexpression of HGF increases β-cell proliferation, insulin production, and glucose tolerance in mice.{53760} Pharmacological inhibition of HGF decreases β-cell mass and induces hyperglycemia and glucose intolerance, markers of gestational diabetes, in pregnant rats. High serum levels of HGF are associated with metastasis, while low serum levels of HGF are positively correlated with progression-free survival and overall survival in lung, gastric, and colon cancers, as well as malignant melanoma.{53759} Intramuscular injection of HGF into ischemic myocardium increases blood flow and prevents myocardial dysfunction in a canine model of myocardial infarction.{53761} Cayman's HGF (human, recombinant) protein can be used in cell-based assay applications. This protein, pro-HGF, which can be further processed into the α and β chains, consists of 697 amino acids, has a calculated molecular weight of 79.7 kDa, and a predicted N-terminus of Glu32 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 90, 60, and 34 kDa for pro-HGF, the heavy α chain, and the light β chain, respectively, due to glycosylation.

-80

-80

Lyophilized from sterile PBS, pH 7.0, with 100 mM arginine and 0.05% Tween 20

365

≥93% estimated by SDS-PAGE