货号:CDX-F0027-G010 | 规格:10 g | 目录价:¥1148 |
货号:CDX-F0027-G025 | 规格:25 g | 目录价:¥2310 |
货号:CDX-F0027-G100 | 规格:100 g | 目录价:¥7014 |
产品详情
产品名称:
Fluorescein diacetate
别名:
FDA; 3,6-Diacetoxyfluoran; Di-O-Acetylfluorescein; Cellstain-FDA; NSC 4726; NSC 667259
产品描述:
FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: lambdaex=494nm, lambdaem=521nm.
宿主来源:
Synthetic.
保存温度:
-20°C
运输温度:
Ambient
预期分子量:
416.38
产品形式:
solid
有效期:
Stable for at least 2 years after receipt when stored at -20°C.
产地:
瑞士
纯度:
>95% (NMR)
CAS号:
596-09-8
分子式:
C24H16O7
溶解度:
Soluble in DMSO, ethanol, water, chloroform or acetone (25mg/ml).
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