FIGURE 1：

CUT&RUN gene browser tracks. CUT&RUN was   performed as described above. Data verifies low non-specific MNase digestion   with the absence of peaks in the IgG track, an expected H3K4me3 profile with   sharp promoter peaks, and broad peaks in heterochromatin regions consistent   with H3K27me3. Image was generated using the Integrative Genomics Viewer   (IGV, Broad Institute).

FIGURE 2：

Size distribution of released chromatin. CUT&RUN   was performed as described above. Excised DNA is highly enriched for   mononucleosomes (peaks at ~300 bp represent 150 bp nucleosomes + sequencing   adapters).

FIGURE 3：

CUT&RUN genome-wide heatmaps. CUT&RUN was   performed as described above. Heatmaps show CUT&RUN signal aligned to   annotated transcription start sites (TSS, +/- 2kb). High and low signal are   ranked by intensity (top to bottom) and colored such that red indicates high   localized enrichment and blue denotes background signal. Gene rows in each   heatmap are aligned and sorted from high to low signal relative to H3K4me3   (middle).

FIGURE 4：

Protein gel data. CUTANATM pAG-MNase (1 µg) was   resolved via SDS-PAGE and stained with Coomassie blue. The migration and   molecular weight of the protein standards are indicated.

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CUTANA™   pAG-MNase for ChIC/CUT&RUN Workflows

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CUTANA™ pAG-MNase   for ChIC/CUT&RUN Workflows

250   Reactions