8-strip Tubes

End Prep   Enzyme

Ligation   Enhancer

SPRIselectreagent   manufactured by

Beckman   Coulter, Inc.

End Prep   Buffer

High   Fidelity 2X PCR

Master Mix

0.1X TE   Buffer

Adapter for   Illumina®

U-Excision   Enzyme

Ligation Mix

i5 and i7   Primers

FIGURE 1：

CUT&RUN   DNA Fragment Size Distribution Analysis. CUT&RUN was  performed as   described above. Library DNA was  analyzed by Agilent TapeStation®   , which  confirmed that mononucleosomes were  predominantly   enriched in CUT&RUN (~300 bp  peaks represent 150 bp nucleosomes +    sequencing adapters). Peaks at ~380 bp  correspond to the   SNAP-CUTANA™ K-MetStat Panel of spike-in controls(EpiCypher 19-1002).

FIGURE 2：

Representative   Gene Browser Tracks. CUT&RUN was performed as described above. A   representative 674 kb window at the SEPTIN5 gene is shown for three   replicates (”Rep”) of IgG and H3K4me3 antibodies, as well as individual   tracks for H3K27me3 and the transcription factor CTCF, demonstrating the   robustness and reproducibility of the workflow with a variety of   targets. Sequencing libraries prepared with the CUTANA CUT&RUN   Library Prep kit produced the expected genomic distribution for each target.   Images were generated using the Integrative Genomics Viewer (IGV, Broad   Institute)

14-1001

CUTANA™   CUT&RUN Library Prep Kit with Primer Set 1

48 Reactions

14-1002

CUTANA™   CUT&RUN Library Prep Kit with Primer Set 2

48 Reactions