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折扣/货期| 货号：CDX-A0668-G025 | 规格：25 g | 目录价：￥1064 |
| 货号：CDX-A0668-G100 | 规格：100 g | 目录价：￥3458 |
产品详情
产品名称:
Acridine Orange hemi(zinc chloride) salt
别名:
3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt; Basic Orange 14; Euchrysin GNY; C.I. 46005; Acridine Orange
产品描述:
Acridine Orange hemi(zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. It is used as a nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. Being cell-permeable, it interacts with DNA and RNA by intercalation or electrostatic attractions respectively. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502nm and an emission maximum at 525nm (green). When acridine orange associates with RNA, the excitation maximum shifts to 460nm (blue), and the emission maximum shifts to 650nm (red). Acridine orange will also enter acidic compartments such as lysosomes where it becomes protonated and sequestered. Within these low pH vesicles, the dye emits red fluorescence when excited by blue light. Thus, acridine orange can be used to visualize primary lysosomes and phagolysosomes that may include products of phagocytosis of apoptotic cells. The dye is often used in epifluorescence microscopy and flow cytometry.It allows for visual detection of nucleic acids on agarose and polyacrylamide gels, can be used for differentiation of dsDNA (green fluorescence) and ssDNA/RNA (orange fluorescence) and as a vitality test for determination of living cells. Spectral data: lambdaEx=502nm, lambdaEm=525nm (green, double strands) / lambdaEx=460nm, lambdaEm=650nm (red, single strands).
宿主来源:
Synthetic
保存温度:
+20°C
运输温度:
Ambient
预期分子量:
369.96
产品形式:
solid
有效期:
Stable for at least 2 years after receipt when stored at RT.
产地:
瑞士
纯度:
>90% (Dye content)
CAS号:
10127-02-3
分子式:
C17H20ClN3 . HCl . 1/2ZnCl2
溶解度:
Soluble in water (1 mg/ml) or ethanol (2mg/ml).
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