褪黑激素(Melatonin，MT)又称为N-乙酰基-5-甲氧基色胺，在松果体中产生，是调节昼夜节律的主要成分。褪黑激素是由色氨酸通过一系列羟基化和甲基化步骤产生的。褪黑素在肝脏中代谢成6-羟基硫酸褪黑素形式后随尿液排出体外。褪黑素合成后，储存在松果体内，交感神经兴奋支配松果体细胞释放褪黑素。褪黑素的分泌具有明显的昼夜节律，白天分泌受抑制，晚上分泌活跃。褪黑素可抑制下丘脑-垂体-性腺轴，使促性腺激素释放激素、促性腺激素、黄体生成素以及卵泡雌激素的含量均减低，并可直接作用于性腺，降低雄激素、雌激素及孕激素的含量。褪黑激素及其代谢产物具有抗氧化和清除自由基的潜力。还有证据表明，它们在活性氧蛋白途径的调节中发挥作用。研究表明，褪黑激素水平与某些癌症的发病率可能存在相关性。唾液可以用来检测褪黑激素水平。与血清或血浆相比，唾液相对容易获得，并且不含有血清和血浆中存在的诸多干扰物质。唾液中的褪黑激素代表了全身循环的水平，尽管确切的比例尚未完全量化，公布的数值范围为血清值的20-50%。褪黑素在癌症、睡眠周期、衰老和其他领域的作用也越来越引起人们的关注，尤其关注褪黑素受体和循环褪黑素的生物利用度，以及褪黑素作为膳食补充剂的使用。
ENZO Melatonin ELISA kit是一种比色竞争法免疫检测试剂盒，可在2小时内得到检测结果。
● 高灵敏度，可检测到低至0.162 ng/ml的褪黑素
● 检测范围广，适用于多种样本
● 高通量，2小时内可获得多达40个样品的结果（带复孔）
● 对包括水果匀浆在内的各种样品进行了验证
产品信息
产品货号 | ENZ-KIT150-0001 |
产品名称 | Melatonin ELISA Kit（褪黑素ELISA试剂盒） |
规格 | 96 wells |
其他名称 | N-acetyl-5-methoxytryptamine |
灵敏度 | 0.162 pg/ml |
检测范围 | 0.08-50 pg/ml |
检测时间 | 2 hours |
样本种属 | Species independent |
样本类型 | Validated specifically for saliva, serum, plasma, and fruit samples |
保存温度 | All components of this kit, except the standard and antibody, can be stored at +4°C. The standard and antibody must be stored at -20°C |
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试剂盒组分 | Goat anti-mouse IgG Microtiter Plate, Assay Buffer 10, Standard, Wash Buffer Concentrate, Antibody, Tracer, Conjugate, TMB Substrate, and Stop Solution |
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| Parallelism of the curves demonstrates that the standard binding characteristics are similar enough to allow the accurate determination of native melatonin levels in diluted samples matrices of animal and plant origin. |
| Typical Melatonin ELISA Data |
| This experiment shows the normal rise and fall of melatonin levels during the course of the day, and this kit’s ability to measure the biological relevant amounts. This experiment shows the normal rise and fall of melatonin levels during the course of the day, and this kit’s ability to measure the biological relevant amounts. |
部分产品文献引用
1. Exogenous SA Applications Alleviate Salinity Stress via Physiological and Biochemical changes in St John’s Wort Plants: E.H. Kwon, et al.; Plants 12, 310 (2023)
2. The circadian system in cystic fibrosis mice is regulated by histone deacetylase 6: E. Barbato, et al.; Am. J. Physiol. Cell Physiol. 323, C1112 (2022)
3. Tubulin Polymerization Promoting Protein Affects the Circadian Timing System in C57Bl/6 Mice: E. Barbato, et al.; J. Circadian Rhythms 19, 5 (2021)
4. Administration of melatonin improves testicular blood flow, circulating hormones, and semen quality in Shiba goats: H. Samir, et al.; Theriogenology 146, 111 (2020), Application(s): Goat plasma
5. Melatonin activates cell death programs for the suppression of uterine leiomyoma cell proliferation: P.H. Lin, et al.; J. Pineal Res. 68, e12620 (2020), Application(s): Mouse serum
6. Melatonin decreases cocaine-induced locomotor activity in pinealectomized rats: S. Barbosa-Méndez & A. Salzar-Juárez; Braz. J. Psychiatry 42, 295 (2020), Application(s): Rat plasma
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