CleanCap® FLuc mRNA (5moU) - (L-7202)
萤火虫荧光素酶mRNA (Trilink cat#L-7202)
产品描述
FLuc mRNA表达一种荧光素酶蛋白，该蛋白最初是从萤火虫体内分离出来的。荧光素酶通常用于动物细胞培养中的基因表达和细胞活性检测。在荧光素底物的存在下能够发光。
该mRNA使用Trilink公司专利加帽法cleancap产生的天然的cap1帽结构，cleancap的加帽效率较高。该mRNA具有PolyA尾巴，且经过5-甲氧基尿苷修饰，针对哺乳动物系统进行了优化，和天然成熟的mRNA相似。
产品细节
产品货号 | L-7202 |
纯化方式 | Passes Agarose Gel Mobility |
长度 | 1929 nucleotides |
碱基组成 | Fully substituted with 5-Methoxy-U |
浓度 | 1.0 mg/mL |
溶液 | 1 mM Sodium Citrate pH 6.4 |
转换因子 | 40 µg/OD260 |
推荐储存条件 | At or below -40°C |
应用 | Reporter Genes |
加帽结构 | AG Start, Cap 1, CleanCap |
其他名称 | CleanCap®Firefly Luciferase mRNA (5-methoxyuridine) |
参考文献：
References
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Herrera, M;Kim, J;Eygeris, Y;Jozic, A;Sahay, G; . Illuminating endosomal escape of polymorphic lipid nanoparticles that boost mRNA delivery
Duran-Mota, JA;Yani, JQ;Almquist, BD;Borr . Polyplex-Loaded Hydrogels for Local Gene Delivery to Human Dermal Fibroblasts
Jansig, E;Geissler, S;Rieckmann, V;Kuenemund, A;Hietel, B;Schenk, M;Wussow, S;Kreideweiss, P;Panzner, S;Reinsch, C;Cynis, H; . Viromers as carriers for mRNA-mediated expression of therapeutic molecules under inflammatory conditions
Lacroix, C;Humanes, A;Coiffier, C;Gigmes, D;Verrier, B;Trimaille, T; . Polylactide-Based Reactive Micelles as a Robust Platform for mRNA Delivery
Ryals, RC;Patel, S;Acosta, C;McKinney, M;Pennesi, ME;Sahay, G; . The effects of PEGylation on LNP based mRNA delivery to the eye
Kim, J;Jozic, A;Sahay, G; . Naturally Derived Membrane Lipids Impact Nanoparticle-Based Messenger RNA Delivery
Gambari, R;Gasparello, J;Fabbri, E;Borgatti, M;Tamanini, A;Finotti, A; . Peptide Nucleic Acids for MicroRNA Targeting
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