炎症小体（inflammasome ），是由胞浆内模式识别受体(PRRs)参与组装的多蛋白复合物,是天然免疫系统的重要组成部分。炎症小体能够识别病原相关分子模式(PAMPs)或者宿主来源的损伤相关分子模式(DAMPs),募集和激活半胱天冬酶-1（Caspase-1）。活化的Caspase-1可切割IL-1β和IL-18前体蛋白,使其产生成熟的细胞因子。炎症小体的活化还能够诱导细胞的炎症坏死(pyroptosis)。
目前已发现的炎症小体主要由NLRP1炎症小体、NLRP3炎症小体、IPAF(NLRC4)炎症小体、NLRP6炎症小体、RIG-I炎症小体和AIM2炎症小体。其中NLRP3炎症小体作为天然免疫的重要组分在多种疾病过程中都发挥了关键作用,包括家族性周期性自身炎症反应、2型糖尿病、阿尔海默茨病和动脉粥样硬化症等。因此,作为炎症反应的核心,NLRP3炎症小体可能为各种炎症性疾病的治疗提供新的靶点 。
| 炎症小体诱导剂 |
产品名称 | 货号 | 描述 | 规格 | 品牌 |
NLRP3炎症小体诱导剂 |
Alum Crystals | tlrl-alk | Aluminium potassium sulfate | 1g | invivogen |
ATP | tlrl-atp | Adenosine 5'-triphosphate disodium salt | 1g | invivogen |
CPPD Crystals | tlrl-cppd | Calcium Pyrophosphate Dihydrate crystals | 5mg | invivogen |
Hemozoin | tlrl-hz | Synthetic heme crystal | 5mg | invivogen |
MSU Crystals | tlrl-msu | Monosodium Urate Crystals | 5mg | invivogen |
Nano-SiO2 | tlrl-sio | Nanoparticles of silica dioxide | 10mg | invivogen |
Nigericin | tlrl-nig | Nigericin, sodium salt | 10/50mg | invivogen |
AIM2炎症小体诱导剂 |
Poly(dA:dT) | tlrl-patn | RIG-I Agonist - CDS Agonist | 200ug/1mg | invivogen |
| 炎症小体抑制剂 |
产品名称 | 货号 | 产品描述 | 规格 | 品牌 |
Bay11-7082 | tlrl-b82 | NLRP3 Inflammasome Inhibitor - IκB-α Inhibitor | 10mg | invivogen |
Glybenclamide | tlrl-gly | NLRP3 Inflammasome Inhibitor - Proton pump inhibitor | 1g | invivogen |
Z-VAD-FMK | tlrl-vad | NLRP3 Inflammasome Inhibitor - Caspase inhibitor | 1mg | invivogen |
 Figure: Mouse caspase-1 is detected in cell culture supernatants by ELISA using caspase-1 (mouse) Matched Pair Detection Set (Prod. No AG-46B-0003). |
| Adipogen公司也可提供多种炎症小体信号相关的研究试剂
定量检测炎症小体的活化
产品名称：Caspase-1 (mouse) Matched Pair Detection Set
产品编号：AG-46B-0003-KI01 规格：1 Set
特 异 性： Detects mouse caspase-1 (p10 and p20 domain).
Does not detect human caspase-1.
反 应 性： Mouse
灵 敏 度： 100pg/ml
检测范围： 0.15ng/ml to 10ng/ml
样本类型： Cell Culture Supernatant |
|
Figure: Mouse caspase-1 (p10) is detected by immunoblotting using anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (Prod. No AG-20B-0044). |
| 用于检测活化的小鼠Caspase-1(p10&p20)的特异性单抗
产品名称：anti-Caspase-1 (p10) (mouse), mAb (Casper-2)
产品编号： AG-20B-0044-C100 100 μg，AG-20B-0044B-C100 Biotin 100 μg
克隆号： Casper-2
亚型： Mouse IgG2a
免疫原： Recombinant mouse caspase-1
应用： WB (1μg/ml)
特异性： Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1. |
| 产品名称 | 产品编号 | 克隆号 | 亚型 | 免疫原 | 应用 | 特异性 |
| anti-Caspase-1 (p20) (mouse), mAb (Casper-1) | AG-20B-0042-C100 100 μg，
AG-20B-0042B-C100 Biotin 100 μg | Casper-1 | Mouse IgG1 | Recombinant mouse caspase-1 | WB (1μg/ml) (see online protocol), IHC (PS), IP | Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1. |
| 产品名称 | 产品编号 | 克隆号 | 亚型 | 免疫原 | 应用 | 特异性 |
| anti-Caspase-1 (p20) (human), mAb (Bally-1) | AG-20B-0048-C100 100 μg | Bally-1 | Mouse IgG1 | Recombinant human caspase-1 | WB (1μg/ml) (see online protocol) | Recognizes endogenous full-length and activated (p20 fragment) human caspase-1. |
| 标准炎症小体信号抗体（standard inflammasome signaling antibodies） |
产品名称 | 产品编号 | 规格 | 品牌 |
anti-Asc, pAb (AL177) | AG-25B-0006-C100 | 100 μg | AdipoGen |
anti-AIM2 (human), mAb (3B10) | AG-20B-0040-C100 | 100 μg | AdipoGen |
anti-Cardif (human), mAb (Adri-1) | AG-20B-0004-C100 | 100 μg | AdipoGen |
anti-MDA5 (human), mAb (Hely-1) | AG-20B-0013-C100 | 100 μg | AdipoGen |
anti-Nod1 (human), pAb (AL184) | AG-25B-0013-C050 | 50 μg | AdipoGen |
anti-NS3 (HCV), mAb (1B6) | AG-20B-0001-C100 | 100 μg | AdipoGen |
anti-NS5B (HCV), mAb (5B-3B1) | AG-20B-0002-C100 | 100 μg | AdipoGen |
anti-NS5B (HCV), mAb (blocking) (5B-12B7) | AG-20B-0003-C100 | 100 μg | AdipoGen |
anti-Pyrin (human), pAb (AL196) | AG-25B-0020-C100 | 100 μg | AdipoGen |
anti-RIG-I, mAb (Alme-1) | AG-20B-0009-C100 | 100 μg | AdipoGen |
anti-RIG-I, mAb (Alme-1) (Biotin) | AG-20B-0009B-C100 | 100 μg | AdipoGen |
| 其他炎症小体信号抗体（other inflammasome signaling antibodies） |
产品名称 | 产品编号 | 规格 | 品牌 |
anti-HMGB1, mAb (rec.) (Giby-1-4) | AG-27B-0002-C100 | 100 μg | AdipoGen |
anti-HMGB1, mAb (rec.) (Giby-1-4) (Biotin) | AG-27B-0002B-C100 | 100 μg | AdipoGen |
anti-NAIP1/2/5 (mouse), mAb (Naipa-1) | AG-20B-0045-C100 | 100 μg | AdipoGen |
anti-NLRP1/NALP1 (human), pAb (AL176) | AG-25B-0005-C100 | 100 μg | AdipoGen |
anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) | AG-20B-0006-C100 | 100 μg | AdipoGen |
anti-NLRP6/NALP6 (human), mAb (Clint-1) | AG-20B-0046-C100 | 100 μg | AdipoGen |
anti-NLRP12/NALP12 (human), pAb (AL236) | AG-25B-0021-C100 | 100 μg | AdipoGen |
| 炎症小体信号相关的蛋白&抗体 |
产品名称 | 产品编号 | 规格 | 品牌 |
IL-1β (human) (rec.) (untagged) | AG-40B-0023-C010 | 10 μg | AdipoGen |
IL-1β (human) (rec.) (untagged) (MultiPack) | AG-40B-0023-3010 | 3 x 10 μg | AdipoGen |
IL-1β (mouse) (rec.) (untagged) | AG-40B-0086-C010 | 10 μg | AdipoGen |
IL-1β (mouse) (rec.) (untagged) (MultiPack) | AG-40B-0086-3010 | 3 x 10 μg | AdipoGen |
IL-1 Receptor Type I (human):Fc (human) (rec.) | AG-40B-0024-C050 | 50 μg | AdipoGen |
IL-1 Receptor Type I (human):Fc (human) (rec.) (MultiPack) | AG-40B-0024-3050 | 3 x 10 μg | AdipoGen |
anti-IL-1α (mouse), mAb (Bamboo-1) | AG-20B-0050-C100 | 100 μg | AdipoGen |
anti-IL-1R2 (mouse), mAb (rec.) (blocking) (Praxy-1) | AG-27B-0011-C100 | 100 μg | AdipoGen |
更多inflammasome产品信息，请联系欣博盛生物，全国服务热线：4006-800-892。