TNFRSF8/CD30 Long Isoform Extracellular Domain (human, recombinant)

D1S166E|Ki-1|Tumor Necrosis Factor Receptor Superfamily Member 8

CD30, also known as tumor necrosis factor receptor superfamily member 8 (TNFRSF8), is a type I transmembrane glycoprotein encoded by TNFRSF8 in humans.{52838,52839} Alternative splicing of the TNFRSF8 pre-mRNA produces one full-length isoform, CD30 long, and two short isoforms that contain truncated transmembrane and/or extracellular domains.{52838,52840} CD30 long exists as a transmembrane monomer and is composed of an extracellular domain containing three cysteine-rich repeats (CRDs), a transmembrane domain, and a cytoplasmic domain that facilitates intracellular signaling.{52839} It is expressed on certain subsets of activated T- or B cells, as well as in a variety of lymphoid neoplasms, including Hodgkin lymphoma and anaplastic large cell lymphomas (ALCLs).{52838} It can also exist in a soluble form, which is produced by proteolytic cleavage of CD30 at the cell surface and is found in the serum.{52838} CD30 is upregulated by mitogens, such as phorbol 12-myristate 13-acetate (TPA; Item No. 10008014), or viral stimulation, including Epstein-Barr virus (EBV) or HIV, as well as by the transcription factor JunB.{52840,52838} Upon interaction with its ligand, TNFSF8/CD30L, which is also expressed on certain subsets of activated lymphocytes, CD30 assembles into a trimer that recruits TNF receptor-associated factor 1 (TRAF1), TRAF2, and TRAF5 to induce NF-κB- or ERK/MAPK-dependent downstream signaling, mediating a variety of cellular processes, including NF-κB activation, cell proliferation and survival, as well as apoptosis.{52838,52839} Neutralization of CD30 with a monoclonal antibody reduces tumor growth in an ALCL patient-derived xenograft (PDX) mouse model.{52841} Serum levels of soluble CD30 (sCD30) are correlated with disease stage in patients with Hodgkin lymphoma or systemic lupus erythematosus (SLE).{52839} Cayman's TNFRSF8/CD30 Long Isoform (human, recombinant) protein can be used for binding assay applications. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of CD30 (amino acids 19-379) fused to His-tagged human IgG1 Fc at its C-terminus, consists of 609 amino acids, has a calculated molecular weight of 66.5 kDa, and a predicted N-terminus of Phe19 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 130 kDa by SDS-PAGE under reducing conditions.

-80

-80

Lyophilized from sterile PBS, pH 7.4

365

≥90% estimated by SDS-PAGE