Tie2/CD202b Extracellular Domain (human, recombinant)

Angiopoietin-1 Receptor|Endothelial Tyrosine Kinase|Tunica Interna Endothelial Cell Kinase 2|Tyrosine-Protein Kinase Receptor TEK

Tunica interna endothelial cell kinase 2 (Tie2), also known as CD202b, is a receptor tyrosine kinase that is encoded by the TEK gene in humans.{60771,60772} It is composed of an extracellular ligand-binding domain containing immunoglobulin, EGF-like, and fibronectin-like repeats, a transmembrane domain, and an intracellular tyrosine kinase domain.{60771} Tie2 is expressed in embryonic and adult endothelial cells, as well as hematopoietic stem cells (HSCs), where it is bound by the growth factors angiopoietin-1, -2, and -4. Upon ligand binding, Tie2 forms homodimers or heterodimers with Tie1, resulting in Tie2 phosphorylation and activation of various signaling pathways, including the PI3K pathway, to promote angiogenesis, endothelial cell survival, and hematopoiesis.{60771,60773} TEK knockout in mice is embryonic lethal with embryos lacking cardiovascular system development and exhibiting vascular hemorrhaging.{60771} Tie2 is upregulated in the vascular endothelium of several cancers, including lung, breast, and prostate cancers, and somatic mutations in TEK result in the development of blue rubber bleb nevus syndrome, a disease characterized by venous malformations and gastrointestinal lesions.{60771,60772} Cayman's Tie2/CD202b Extracellular Domain (human, recombinant) protein can be used for ELISA and Western blot (WB) applications. This protein is a disulfide-linked homodimer. The reduced monomer, composed of Tie2 (amino acids 23-745) fused to His-tagged human IgG1 Fc at its C-terminus, consists of 970 amino acids, has a calculated molecular weight of 108.5 kDa, and a predicted N-terminus of Ala23 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 130.7 kDa by SDS-PAGE under reducing conditions.

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-80

Lyophilized from sterile PBS, pH 7.4

365

≥90% estimated by SDS-PAGE