货号:16-0027 | 规格:50 µg | 目录价:¥6900 |
产品详情
产品名称:
Tailless Nucleosomes, Recombinant Human, Biotinylated
产品描述:
Monoucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805). Histones were assembled into octamers and wrapped with 147 base pairs of 5’ biotinylated 601 Sequence DNA, originally discovered by Lowary and Widom [1].After assembly, histone tails were enzymatically removed. This product is ideal for use as a negative control in binding assays and pull-down experiments.
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
Purified recombinant tailless nucleosomes (50 µg protein + DNA total mass, 25 µg protein by mass) in 46.9 µL of 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, 20% glycerol. Molarity = 5.82 µM. MW = 183,160.5 Da (theoretical).
产地:
美国
生产商:
EpiCypher
上一个:Dinucleosomes, Recombinant Human, Non-Biotinylated
下一个:Mononucleosomes, Recombinant Human, Desthiobiotinylated
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