货号:SA-5488-1 | 规格:1 mg | 目录价:¥3084 |
产品详情
产品名称:
Streptavidin, DyLight™ 488, 1 mg
产品描述:
DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.DyLight® dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight® conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight® conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents. Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometryHighly purified and possess very low non-specific binding propertiesExtremely high affinity for biotinAdvantages of DyLight® dyes include greater photostability, pH independence, and brighter fluorescenceExcitation at 493 nmEmission at 518 nmColor: Green
实验应用:
Immunofluorescence,In situ hybridization,Flow Cytometry/Cell Separation
推荐稀释度:
Recommended concentration range for use5-30µg/ml
Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
偶联:
DyLight 488
产品浓度:
1.0 mg/ml active conjugate
保存温度:
2-8 °C
运输温度:
Ambient
产品形式:
Concentrate
存储溶液:
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
产地:
美国
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