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折扣/货期| 货号：SA-5008-1 | 规格：1 mg | 目录价：￥3002 |
产品详情
产品名称:
Streptavidin, AMCA, 1 mg
产品描述:
AMCA Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence. Most paraffin-embedded tissues have little autofluorescence under those conditions. AMCA Streptavidin may provide an advantage in many labeling situations over conjugates of fluorescein and related dyes. AMCA Streptavidin can be used on frozen or paraffin-embedded sections as a single label or in combination with other fluorescent conjugates for multiple labeling studies.
实验应用:
Immunofluorescence,In situ hybridization,Flow Cytometry/Cell Separation
推荐稀释度:
For diluting this product, we recommend a HEPES- or biocarbonate-buffered saline
solution, approximately pH 8.2.Avoid using RPMI 1640 or other biotin-containing
solutions as diluents.Serum also can contain biotin and should not be added to diluents.
The recommended concentration range for use is 10-30µg/ml.
偶联:
AMCA
产品浓度:
1.0 mg/ml
保存温度:
2° - 8°C – Do Not Freeze
运输温度:
Ambient
产品形式:
Concentrate
存储溶液:
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
产地:
美国
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