SARS-CoV-2 Papain-like Protease (recombinant)

2019-nCoV PLpro|COVID-19-PLpro|SARS-CoV-2 PLpro|Severe Acute Respiratory Syndrome Coronavirus 2 Papain-like Protease

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} The SARS-CoV-2 papain-like protease (PLpro) is encoded within the non-structural protein 3 (nsp3) region of ORF1ab. It cleaves non-structural proteins 1-3, which, together with non-structural proteins 4-16, form the replicase complex. In addition, it reverses certain post-translational modifications, preferentially removing interferon-stimulated gene product 15 (ISG15), but also removing ubiquitin, from host proteins with both actions leading to suppression of the host innate immune response.{54466,54467} SARS-CoV-2 PLpro decreases IFN-α-induced ISGylation of IFN regulatory factor 3 (IRF3) in A549 cells and inhibits the type I IFN response.{54467} Inhibition of SARS-CoV-2 PLpro activity reduces SARS-CoV-2 replication and viral particle release from infected cells in vitro and increases the level of IRF3 ISGylation. Cayman’s SARS-CoV-2 Papain-like Protease (recombinant) protein can be used for enzyme activity assays. This protein consists of 324 amino acids and has a calculated molecular weight of 36.8 kDa.

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Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride

365

≥90% estimated by SDS-PAGE