九游会j9网站首页,j9九游会首页登录,j9九游会ag真人官网

您好,欢迎光临九游会j9网站首页商城!
服务热线:400 680 0892
Aleuria Aurantia Lectin (AAL), Agarose bound, 2 ml/橙黄网孢盘菌凝集素,琼脂糖交联
品牌: Vectorlabs
产品货号: AL-1393-2
规格: 2 ml

报价:
折后价:

数量: - +
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
  • 产品名称:
    Aleuria Aurantia Lectin (AAL), Agarose bound, 2 ml/橙黄网孢盘菌凝集素,琼脂糖交联
  • 别名:
    橙黄网孢盘菌凝集素,琼脂糖交联
  • 产品描述:
    Aleuria aurantia lectin AAL is a dimer of two identical subunits of about 36 kDa each. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acidsAgarose bound* Aleuria Aurantia lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Inhibiting/Eluting Sugar: 100 mM L-fucose*2 mg lectin/ml gel
  • 反应种属:
  • 宿主来源:
  • 实验应用:
    Glycobiology,Affinity Chromatography
  • 靶标/特异性:
  • 同种型:
  • 推荐稀释度:
    Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  • 免疫原:
  • 免疫原种属:
  • 克隆性:
  • 克隆号:
  • 纯化方式:
  • 偶联:
    Agarose
  • 产品浓度:
  • 表达系统:
  • 阳性对照:
  • 保存温度:
    2° - 8°C – Do Not Freeze
  • 运输温度:
    Ambient
  • 细胞定位:
  • 预期分子量:
  • 产品形式:
  • 存储溶液:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.08% sodium azide
  • 有效期:
  • 产地:
    美国
  • 外观:
  • 生产商:
  • 功能与背景:
  • URL:
  • 灵敏度:
  • 检测范围:
  • 检测方法:
  • 样本类型:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.08% sodium azide
  • 产品特点:
  • 纯度:
  • 生物活性:
  • 内毒素水平:
  • 来源:
  • 表达方式:
  • Accession #:
  • CAS号:
  • 分子式:
  • 溶解度:
  • PubChem CID:
关注欣博盛公众号
Copyright ©2021 - 2023 深圳九游会j9网站首页有限公司
  网站地图
犀牛云提供企业云服务
九游会j9网站首页