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    PPARδ Transcription Factor Assay Kit
    货号:10006914 规格:96 wells 目录价:¥6902
    产品详情
    产品名称:
    PPARδ Transcription Factor Assay Kit
    别名:
    Peroxisome Proliferator-activated Receptor δ Transcription Factor Assay Kit
    产品描述:
    Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.{10561} Cayman’s PPARδ Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARδ binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE from which PPARδ is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
    保存温度:
    -80
    运输温度:
    -80
    有效期:
    365
    来源:
    Animal/Bovine|Animal/Rabbit|Animal/Goat
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