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    Epicypher新品 | 核酸提取缓冲液

    2023-11-22
    浏览次数: 114


    Epicypher新品 | 核酸提取缓冲液


    CUTANA™ 细胞核提取缓冲液是CUT&RUN和CUT&Tag实验中,用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型:

    mouse NIH3T3 fibroblast cells

    human A549 non-small cell lung cancer (NSCLC) cells

    human K562 leukemia cells

    human NCI-H1299 non-small cell lung cancer (NSCLS cells)

    human bone marrow derived macrophages

    human TIG-1 fetal lung cells

    human monocyte derived macrophages

    human LoVo colorectal cancer cells

    human MV-4-11 macrophage cells

    human LNCaP prostate carcinoma cells

    human SUM149 triple negative breast cancer (TNBC) cells

    human renal primary cells

    human GM24385 B-lymphocyte (aka HG002) cells

    human peripheral blood mononuclear cells (PBMCs)

    human MCF7 breast cancer cells

    human intestinal tissue

    human MDA-MB-231 breast cancer cells

    human HEPM embryonic cells

    human SK-MEL-2 melanoma cells


     

    只需将试剂盒中的Pre-Nuclei Extraction Buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。CUTANA™ 细胞核提取缓冲液针对CUT&RUN和CUT&Tag实验流程进行了优化,从而获得高质量的细胞核,助力您的基因组图谱分析。

     

    试剂盒组成

    组分

    货号

    CUTANA™ Pre-Nuclei Extraction Buffer      

    21-1026a     

    1 M Spermidine

    21-1026b


    保存条件

    将Pre-Nuclei Extraction Buffer 4ºC储存, 1 M亚精胺-20ºC储存。

    自收到之日起可稳定储存6个月。


    验证数据

    Epicypher新品 | 核酸提取缓冲液

    Figure 1: Nuclei Extraction

    Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.


    订购详情

    货号

    产品名称

    规格

    21-1026

    CUTANA™ Nuclei Extraction Buffer

    100 Reactions

     



    Epicypher热销产品——CUTANA™ pAG-Tn5 for CUT&Tag

    如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物 

    全国服务热线: 4006-800-892       邮箱: market@qckc0531.com 

    深圳: 0755-26755892         北京: 010-88594029            

    广州:020-87615159           上海: 021-34613729

    代理品牌网站: www.nobiosescience.com 

    自主品牌网站: www.neobiosescience.net

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