官网链接
折扣/货期| 货号：700930 | 规格：96 wells | 目录价：￥6258 |
产品详情
产品名称:
MitoCheck® Complex I Activity Assay Kit
产品描述:
Complex I (NADH dehydrogenase/NADH:ubiquinone oxidoreductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocations of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosesmotic proton gradient, which is required for oxidative phosphorylation. Cayman's MitoCheck® Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I. For the use of this kit with other types of tissue mitochondria, please see Cayman's MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).
保存温度:
-80
运输温度:
-80
有效期:
365
来源:
Animal/Bovine
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