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折扣/货期| 货号：B-1145-5 | 规格：5 mg | 目录价：￥3505 |
产品详情
产品名称:
Erythrina Cristagalli Lectin (ECL, ECA), Biotinylated, 5 mg
产品描述:
Erythrina cristagalli lectin ECL, ECA. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins. This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request or on our website). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.Biotinylated Erythrina cristagalli lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.
实验应用:
Immunohistochemistry / Immunocytochemistry,Immunofluorescence,Blotting Applications,Elispot,ELISAs,Glycobiology
推荐稀释度:
or most applications we recommend a freshly prepared working solution of
5-20µg/ml in the above buffer.
偶联:
Biotinylated
产品浓度:
5 mg active conjugate/ml
保存温度:
2-8 °C
运输温度:
Ambient
存储溶液:
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, 0.08% sodium azide.
产地:
美国
上一个:Phaseolus Vulgaris Erythroagglutinin (PHA-E), Biotinylated, 2 mg
下一个:Jacalin, Biotinylated, 5 mg
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