Citrullinated Histone H2B (human, recombinant)

H2BC1|HIST1H2BA|hTSH2B|TH2B|TSH2B.1

Citrullinated Histone H2B Type 1-A is a citrullinated form of human histone H2B type 1-A (hTSH2B), a testis- and sperm-specific variant of the histone H2B protein that is encoded by H2BC1, previously known as HIST1H2BA.{53231,53236} hTSH2B shares 85% identity with human somatic H2B.1, with the majority of amino acid differences between hTSH2B and somatic H2B occurring at the N-terminal end of the protein.{53231} H2BC1 is expressed in testis, and protein levels of hTSH2B are elevated in early round spermatids, decreasing over the course of spermiogenesis, with hTSH2B present in approximately 20% of mature spermatozoa.{53231,53232} hTSH2B is enriched at promoter regions of genes important for sperm biology, capacitation, and fertilization and localizes to the basal nuclear area in mature spermatozoa.{53231,53233} When incubated in Xenopus egg extracts, hTSH2B-positive human sperm cells decondense more rapidly and have increased long nuclear axis lengths compared with hTSH2B-negative sperm cells.{53234} Mice lacking the genes encoding the murine homolog of hTSH2B (TH2B), as well as the histone variant TH2A (Th2a-/-Th2b-/- mice), are sterile and have spermatogenesis defects.{53235} This product contains purified histone H2B type 1-A (human, recombinant) (Item No. 11081) that has been modified with PAD4 enzyme, which is subsequently depleted by affinity chromatography. Cayman's Citrullinated Histone H2B Type 1-A (human, recombinant) can be used for Western blot and ELISA applications.

-80

-80

A solution in water

365

≥90% estimated by SDS-PAGE