BMP2 (recombinant)

Bone Morphogenic Protein 2

Bone morphogenetic protein 2 (BMP2) is a member of the TGF-β superfamily of growth factors that has roles in osteogenesis and embryogenesis.{54170} It is composed of an N-terminal prodomain that regulates BMP2 folding and secretion and a C-terminal mature domain that contains the receptor binding region. BMP2 is expressed by chondrocytes, osteocytes, osteoblasts, and vascular endothelial cells and is synthesized in the cytosol as a proprotein dimer.{54171,54172} Cleavage of the proprotein dimer by proprotein convertase subtilisin kexins (PCSKs) generates the N- and C-terminal domains, which remain non-covalently associated and homodimerize or heterodimerize with BMP4 or BMP7 prior to secretion.{54171} BMP2 preferentially binds to BMP type 1 receptors, also known as activin receptor-like kinases (ALKs), which are expressed by mesenchymal or hematopoietic stem cells, chondrocytes, osteoblasts, osteoclasts, and myoblasts, and activates SMAD-dependent and -independent gene transcription.{54170,54173} BMP2 induces production of collagen type I α and alkaline phosphatase, a marker of bone formation, as well as induces differentiation and proliferation of bone marrow-derived mesenchymal stem cells in vitro.{54174} Genome-wide deletion of Bmp2 in mice induces amnion, chorion, and cardiac developmental defects and is embryonic lethal.{54170} Limb-specific Bmp2 conditional knockout mice exhibit spontaneous fractures and impaired fracture healing.{54175} Serum and synovial fluid BMP2 levels are increased in patients with osteoarthritis and are positively correlated with disease severity.{54176} Formulations containing BMP2 have been used in treatment of long bone fractures. Cayman's BMP2 (recombinant) protein can be used for cell-based assay applications. This protein consists of 115 amino acids that correspond to the mature form of human, canine, mouse, rat, and rhesus BMP2 and has a calculated molecular weight of 13 kDa. By SDS-PAGE, under non-reducing conditions, the protein has apparent molecular weights of approximately 13 and 25 kDa, corresponding to the monomer and dimer, respectively.

-80

-80

Lyophilized from sterile 30% CAN and 0.1% TFA, pH 2.9

365

≥95% estimated by SDS-PAGE