Figure 1:Representative SNAP-ChIP-seq   results: Cumulative histogram plot and heatmap of signal intensity   depict H3K27ac ChIP-seq data aligned to annotated transcription start sites   (TSS, +/- 3.0 kb; left). Two representative genomic regions depicting H3K27ac   peak structure and distribution are shown (right).

Figure 2: SNAP-ChIP-qPCR data. Histone H3K27ac antibody   (3 µg) was tested in a native ChIP experiment using chromatin from K562 cells   (3 µg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher 19-3001) spiked-in   prior to micrococcal nuclease digestion. Specificity (left y-axis) was   determined by qPCR for the DNA barcodes corresponding to modified nucleosomes   in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency   (right yaxis; log scale) and indicates percentage of the target   immunoprecipitated relative to input. Error bars represent mean ± SEM in   replicate ChIP experiments.

13-0045

Histone   H3K27ac Antibody, SNAP-ChIP® Certified

50 µg